Mc Intosh and Filde's Anaerobic Jar - Method of Use

Method of Use

  • First:
  1. The culture: The culture media are placed inside the jar, stacked up one on the other, and
  2. Indicator system: Pseudomonas aeruginosa, inoculated on to a nutrient agar plate is kept inside the jar along with the other plates. This bacteria need oxygen to grow (aerobic). A growth free culture plate at the end of the process indicates a successful anaerobiosis. However, P. aeruginosa possesses a denitrification pathway. If nitrate is present in the media, P. aeruginosa may still grow under anaerobic conditions.
  • Second:

6/7ths of the air inside is pumped out and replaced with either unmixed Hydrogen or as a 10%CO2+90%H2 mixture. The catalyst (Palladium) acts and the oxygen is used up in forming water with the hydrogen. The manometer registers this as a fall in the internal pressure of the jar.

  • Third:

Hydrogen is pumped in to fill up the jar so that the pressure inside equals atmospheric pressure. The jar is now incubated at desired temperature settings.

Read more about this topic:  Mc Intosh And Filde's Anaerobic Jar

Famous quotes containing the words method of and/or method:

    “Methinks the human method of expression by sound of tongue is very elementary, & ought to be substituted for some ingenious invention which should be able to give vent to at least six coherent sentences at once.”
    —Virginia Woolf (1882–1941)

    “Frankly, I adore your catchy slogan, “Adoption, not Abortion,” although no one has been able to figure out, even with expert counseling, how to use adoption as a method of birth control, or at what time of the month it is most effective.”
    —Barbara Ehrenreich (b. 1941)