Mayberrians
- Ralph Barton (Charles Lampkin), Friend of Sam Jones (RFD)
- Ernest T. Bass (Howard Morris), a bothersome trouble maker fond of throwing rocks
- Emma Brand (Cheerio Meredith ), serial jaywalker and hypochondriac
- Emmett Clark (Paul Hartman), Fix-it shop owner
- Otis Campbell (Hal Smith), town drunk and a descendant of a Revolutionary War hero
- Rita Campbell (Dorothy Neumann), Otis Campbell's wife
- Old Man Crowley (Burt Mustin)
- Helen Crump (Aneta Corsaut), a Kansas transplant, Opie's teacher and Andy's girlfriend (later appearing as his wife in Mayberry R.F.D. spinoff series)
- The Darlings, a hillbilly family that plays bluegrass music with Andy in several episodes
- Clara Edwards (Hope Summers), Aunt Bee's best friend
- Warren Ferguson (Jack Burns), a Boston transplant, replacement Deputy (even more inept than Fife)
- Barney Fife (Don Knotts), Andy's lifelong friend (originally portrayed as his cousin in the pilot episode), Deputy Sheriff, later a detective with the Raleigh, NC police department
- Charlie Foley (Frank Ferguson), grocer
- Harold Grigsby (Kelly Thordsen), owns the saw mill
- Orville Hendricks (Woodrow Chambliss), butter and egg man from Mount Pilot
- Evan Hendricks (Bobby Diamond), wild driving son of Orville, known to break an egg or two.
- Mike Jones (Buddy Foster), son of Sam Jones (RFD)
- Sam Jones (Ken Berry), farmer (RFD) and single parent and widower like Andy, later new Sherriff of Mayberry in Mayberry R.F.D. spinoff series
- Juanita, a waitress at the Bluebird Diner, who is never seen (flirts with Barney over the phone)
- Floyd Lawson (Howard McNear), barber at Floyd's Barber Shop
- Jim Lindsey (James Best), talented guitarist who joins Bobby Fleet and His Band with a Beat
- Leon (Clint Howard), a toddler in a cowboy outfit who wandered the streets of Mayberry
- Bert Miller (Sterling Holloway), peddler
- Orville Monroe (Jonathan Hole ), undertaker
- Mayor Pike (Dick Elliott) mayor in first two seasons of show (1960–61)
- Goober Pyle (George Lindsey), auto mechanic at Wally's Garage and Gas Station (later service station owner)
- Gomer Pyle (Jim Nabors), service station attendant at Wally's Garage for 3 seasons (1962–64) (left Mayberry to join the Marine Corps in Gomer Pyle, U.S.M.C. spinoff series)
- Sarah, the often spoken to, but never seen, telephone operator
- Skippy (Joyce Jameson) and Daphne (Jean Carson), the Fun Girls
- Howard Sprague (Jack Dodson), County Clerk
- Roy Stoner (Parley Baer), Mayor of Mayberry for 3 seasons (1962–64), replacing Mayor Pike
- Millie Swanson (Arlene Golonka), bakery clerk (RFD)
- Andy Taylor (Andy Griffith), sheriff and widowed father
- Beatrice Taylor (Frances Bavier), Andy's aunt, known as Aunt Bee
- Opie Taylor (Ronny Howard), Andy's son
- Thelma Lou (Betty Lynn), Barney's sweetheart, occupation unknown
- Ellie Walker (Elinor Donahue), pharmacist and one time love interest of Andy
- Ben Weaver (Will Wright), the flint-hearted store owner and landlord
- Mrs. Wiley (Doris Packer), genealogy researcher, called Ernest T. Bass "a creature"
Read more about this topic: Mayberry