Mashiro, a Japanese surname, may refer to:
- Mashiro Kazahana or Mashiro Blan de Windbloom, a fictional characters in My-HiME and My-Otome
- Mashiro Kuna, a fictional character in Bleach
- Moritaka Mashiro, a fictional character in Bakuman
- Rima Mashiro, a fictional character in Shugo Chara!