Marlene may refer to:
- Marlene (1984 film)
- Marlene (2000 film)
- Marlene (given name), people with the given name Marlene
- "Marlene", a song by Todd Rundgren from the album Something/Anything?
- "Marlene on the Wall", a song by Suzanne Vega from the album Suzanne Vega (album)