Steps For MAS
Generally the first step is to map the gene or quantitative trait locus (QTL) of interest first by using different techniques and then use this information for marker assisted selection. Generally, the markers to be used should be close to gene of interest (<5 recombination unit or cM) in order to ensure that only minor fraction of the selected individuals will be recombinants. Generally, not only a single marker but rather two markers are used in order to reduce the chances of an error due to homologous recombination. For example, if two flanking markers are used at same time with an interval between them of approximately 20cM, there is higher probability (99%) for recovery of the target gene.
Read more about this topic: Marker Assisted Selection
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“They were steps for me, and I climbed up over them—to do so I needed to go on past them. But they supposed I wanted to settle down on them.”
—Friedrich Nietzsche (1844–1900)