MAFF is a four-letter acronym that may refer to:
- MAFF (gene), a transcription factor
- Ministry of Agriculture, Forestry and Fisheries, Cambodia
- Ministry of Agriculture, Forestry and Fisheries, one of ministries of Japanese government.
- Ministry of Agriculture, Fisheries and Food (United Kingdom), a former department of UK government, replaced in 2001.
- Mozilla Archive Format file, a format for archiving web pages and also an add-on of the same name for Mozilla Firefox