Macduff may refer to:
- Macduff (Macbeth), the character in Shakespeare's Macbeth
- Macduff, Aberdeenshire, the former burgh which is now within the Aberdeenshire council area of Scotland
- the Clan MacDuff, a Scottish clan
- MacDuff, Dana, American film producer
- MacDuff, Tyler, American actor
- Ricard MacDuff, a character from the Douglas Adams book series Dirk Gently's Holistic Detective Agency.