Preparation
The following is a common method for the preparation of 1 liter of LB:
- Measure out the following:
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- Suspend the solids in ~800 ml of distilled or deionized water.
- Add further distilled water or deionized water, in a measuring cylinder to ensure accuracy, to make a total of 1 liter.
- Autoclave at 121 °C for 20 mins.
- After cooling, swirl the flask to ensure mixing, and the LB is ready for use.
Read more about this topic: Lysogeny Broth
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