Lyne Bessette (born 10 March 1975, Knowlton, Quebec) is a professional bicycle racer from Quebec, Canada. She was a member of the Canadian Olympic team in 2000 and 2004. She won the Tour de l'Aude Feminin in 1999 and 2001 and the Women's Challenge in 2001. She is married to cyclist Tim Johnson.
Bessette was the pilot for Robbi Weldon's gold medal win at the 2012 Summer Paralympics Women's road race B, winning gold.