Lyles is a surname, and may refer to:
- A. C. Lyles (born 1918), American film producer
- Aubrey Lyles (1883–1932), American songwriter, lyricist, and vaudeville performer
- Jordan Lyles, American baseball player for the Houston Astros
- Kathryn Lyles, American stage actor
- Lenny Lyles (born 1936), American football player
- Lester Lyles (born 1946), United States Air Force general
- Lester Lyles (American football) (born 1962), American football player
- Nate Lyles (born 1985), American football player
- Robert Lyles (born 1961), American football player