Biology and Ecology
- Archibald Macallum (B.A. 1880) – Biochemist and founder of the National Research Council of Canada
- J. Playfair McMurrich (M.A. 1881) – Zoologist and academic, winner of the Flavelle Medal, former president of the Royal Society of Canada and the American Association for the Advancement of Science
- Charles E. Saunders (B.A. 1888) – Agronomist and inventor of Marquis wheat
- Archibald Gowanlock Huntsman (B.A. 1905, professor of marine zoology 1927–54) – Fisheries biologist, invented the fast freezing of fish fillets, recipient of the Flavelle Medal, former president of the Royal Society of Canada
- Sanford Jackson (B.Sc., M.Sc., Ph.D) – Prominent biochemist, former biochemist-in-chief at the Toronto Hospital for Sick Children, inventor of the bilirubinometer
- C. S. Holling (B.A., M.Sc. 1952) – Ecologist and pioneer in ecological economics, director of the International Institute for Applied Systems Analysis in Vienna
- Anne Zeller (M.A. 1971, Ph.D. 1978) – Physical anthropologist specialized in the study of primates
- William E. Rees (Ph.D.) – Ecologist, professor of ecology at the University of British Columbia, origininated the ecological footprint concept and co-developed the method
- Helen Rodd (M.Sc. 1982, associate professor 1998–) – Zoologist, recipient of the Premier's Research Excellence Award
- Jan Conn (Ph.D. 1987) – Geneticist and poet, her book South of the Tudo Bem Cafe shortlisted for the Pat Lowther Award
- Cheryl Arrowsmith (Ph.D.) – Structural biologist, Chief Scientist at the Toronto lab of the Structural Genomics Consortium
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