Ecuador
- Ambato
- Santa Fe, Spain (1990)
- Cuenca
- Cusco, Peru (2000)
- Tempe, Arizona, United States (2009)
- Guayaquil
- Santiago, Chile
- Houston, Texas, United States (1987)
- Genoa, Italy
- Ibarra
- Machu Picchu, Peru
- Winchester, Kentucky, United States
- La Concepción
- Povegliano Veronese, Italy
- Quito
- Coral Gables, Florida, United States
- Louisville, Kentucky, United States (1962)
- Madrid, Spain
- Managua, Nicaragua
- Saint Paul, Minnesota, United States
- Sucre, Bolivia
- Toronto, Ontario, Canada
- Santa Cruz Island (Galápagos)
- Seabrook, Texas, United States
Read more about this topic: List Of Twin Towns And Sister Cities In South America