Chile
- Ancud
- Concepción, Paraguay
- Antofagasta
- Corvallis, Oregon, United States
- Split, Croatia
- Tongling, China
- Arica
- Concepción, Paraguay
- Casablanca Valley
- Napa County, California, United States
- Coquimbo (Region)
- San Juan (Province), Argentina (2005)
- Concepción
- La Plata, Argentina (1993)
- - Cascavel, Brazil
- - Bethlehem, Palestinian National Authority
- - Guayaquil, Ecuador
- - Rosario, Argentina
- Iquique
- Miami-Dade County, Florida, United States
- São Borja, Brazil
- La Serena
- Tenri (天理市), Japan (1966)
- Kraków, Poland (1995)
- Millbrae, California, United States
- Hilo, Hawai'i, United States
- Lo Barnechea
- Tyler, Texas, United States
- Melipilla
- Atibaia, Brazil
- Ubatuba, Brazil
- Itajaí, Brazil
- Osorno
- Blumenau, Brazil
- Pucon
- Lake Oswego, Oregon, United States
- Puerto Montt
- Puerto Madryn, Argentina (1994)
- Qingdao, China (1999)
- Puerto Varas
- San Carlos de Bariloche, Argentina (1993)
- Punta Arenas
- Bellingham, Washington, United States (1996)
- Ushuaia, Argentina (1995)
- Quilpué
- Bondy, France
- Montpellier, France
- San Esteban
- Castellalto, Italy
- Santiago
- Buenos Aires, Argentina (1992)
- São Paulo, Brazil (1998)
- Tokyo, Japan
- Guayaquil, Ecuador
- Riga, Latvia
- Warsaw, Poland
- Ankara, Turkey
- New York, United States
- Paris, France (1997)
- London, UK
- Minneapolis, Minnesota, United States (1961)
- Miami, Florida, United States
- Rapa Nui
- Maui County, Hawaii, United States
- Talcahuano
- Bahía Blanca, Argentina (1995)
- Temuco
- Santiago de Compostela, Spain
- Stavropol, Russia
- Valdivia
- Neuquén, Argentina (2003)
- Hamburg, Germany (1920)
- Cluj-Napoca, Romania
- Tacoma, Washington, United States
- Mount Pleasant, Michigan, United States
- Valparaíso
- Osaka, Japan (2001)
- Barcelona, Spain (2001)
- New York, United States
- Córdoba, Argentina (1993)
- Long Beach, California, United States
- Novorossiysk, Russia
- San Francisco, United States
- Rosario, Argentina (1997)
- Shanghai, China (2001)
- Villarrica
- Magog, Quebec, Canada
- Viña del Mar
- Guangzhou, People's Republic of China
- İzmir, Turkey
- Mar del Plata, Argentina (2001)
- Sausalito, California, United States
Read more about this topic: List Of Twin Towns And Sister Cities In South America