Ligature may refer to:
- Ligature (medicine)
- Ligature (music), a characteristic notation style of the Medieval and Renaissance periods of music history
- Ligature (musical instrument), a device used to attach a reed to the mouthpiece of a woodwind instrument
- Elastic Ligature (orthodontic)
- Typographic ligature - the joining of two glyphs into one
- Ligature strangulation
- Ligature Design Symposium (University of Florida)