Overview
| Grouping | Name | Description | Played by |
|---|---|---|---|
| The FBI/DEA | Special Agent Dale Cooper | Investigates the murder of Laura Palmer using unorthodox methods, falls in love with Twin Peaks and all its rural life, fascinated with the paranormal. He practices Buddhism. | Kyle MacLachlan |
| Albert Rosenfield | Abrasive forensics expert, whose arrogant façade hides a spiritual nature | Miguel Ferrer | |
| Chester Desmond | Investigates murder of Teresa Banks, mysteriously disappears | Chris Isaak | |
| Sam Stanley | Forensics specialist, assists Desmond in the investigation of Teresa Banks' murder | Kiefer Sutherland | |
| Phillip Jeffries | Long-lost FBI agent, who knows of the Lodges and their inhabitants | David Bowie | |
| Roger Hardy | Agent working for Internal Affairs who temporarily suspends Cooper from the FBI. | Clarence Williams III | |
| Gordon Cole | Regional Bureau Chief, known to shout because he is hard-of-hearing | David Lynch | |
| Dennis/Denise Bryson | Cross-dressing DEA agent who investigates drug allegations against Dale Cooper | David Duchovny | |
| Sheriff's Department | Sheriff Harry S. Truman | Lover of Josie Packard and friend of Cooper. Helps him solve the Laura Palmer case. | Michael Ontkean |
| Deputy Andy Brennan | Dorky deputy, lover of Lucy | Harry Goaz | |
| Deputy Hawk | Native American, expert tracker | Michael Horse | |
| Lucy Moran | Receptionist, on and off lover of Deputy Brennan | Kimmy Robertson | |
| Cappy | Young, male assistant at the police station | Ron Kirk | |
| Palmer family | Laura Palmer | Murder victim and the center of the story, linked to almost everyone in Twin Peaks | Sheryl Lee |
| Leland Palmer | Laura's father, known for his compulsive singing and dancing, lawyer | Ray Wise | |
| Sarah Palmer | Laura's psychic mother | Grace Zabriskie | |
| Maddy Ferguson | Laura's nearly identical cousin | Sheryl Lee | |
| Hayward family | Doctor William Hayward | Doctor of Twin Peaks, assists on the autopsy of Laura Palmer | Warren Frost |
| Eileen Hayward | Doctor Hayward's wheelchair-using wife | Mary Jo Deschanel | |
| Donna Hayward | Laura's best friend, lover of James Hurley | Lara Flynn Boyle/Moira Kelly | |
| Harriet Hayward | Donna's younger sister | Jessica Wallenfels | |
| Gersten Hayward | Donna's youngest sister | Alicia Witt | |
| Horne family | Ben Horne | Wealthy businessman, owns Great Northern Hotel, Horne's Department Store and a brothel on the Canadian border | Richard Beymer |
| Jerry Horne | Ben's playboy brother and business partner | David Patrick Kelly | |
| Sylvia Horne | Ben's constantly angry wife | Jan D'Arcy | |
| Audrey Horne | Ben's sultry teenage daughter | Sherilyn Fenn | |
| Johnny Horne | Ben's mentally handicapped son, tutored by Laura | Robert Davenport/Robert Bauer | |
| Packard/Martell Family | Josie Packard | Widowed sawmill owner with a dark past, lover of Sheriff Truman, tutored by Laura | Joan Chen |
| Andrew Packard | Late owner of the Packard Sawmill and husband of Josie | Dan O'Herlihy | |
| Catherine Martell | Sister of Andrew, schemes with secret lover Ben Horne to burn down the sawmill | Piper Laurie | |
| Pete Martell | Long-suffering husband of Catherine, fishing and chess enthusiast, found Laura's body | Jack Nance | |
| Briggs family | Major Garland Briggs | Intelligent and gifted Air Force officer involved in Project Blue Book, deep-space monitoring, and the woods surrounding Twin Peaks | Don S. Davis |
| Betty Briggs | Unassuming and loving wife and mother | Charlotte Stewart | |
| Bobby Briggs | Rebellious teenager, captain of the football team, boyfriend of Laura Palmer, secret lover of Shelly Johnson | Dana Ashbrook | |
| Hurley/Jennings triangle | Big Ed Hurley | Owner of Big Ed's Gas Farm, secret lover of Norma Jennings | Everett McGill |
| Nadine Hurley | Ed's one-eyed, super-strong, curtain drape-obsessed wife | Wendy Robie | |
| James Hurley | Ed's nephew, secret lover of Laura Palmer, later lover of Donna Hayward | James Marshall | |
| Norma Jennings | Double-R Diner owner, lover of Big Ed Hurley, organizer of Meals on Wheels with Laura | Peggy Lipton | |
| Hank Jennings | Norma's husband, criminal on parole, conspirator with Josie Packard | Chris Mulkey | |
| Annie Blackburn | Younger sister of Norma, an ex-nun with a troubled past | Heather Graham | |
| The Johnsons | Leo Johnson | Trucker and drug-runner, had a sexual relationship with Laura Palmer | Eric Da Re |
| Shelly Johnson | Abused young wife of Leo, waitress at Norma's diner, secret lover of Bobby Briggs | Mädchen Amick | |
| One Eyed Jack's/ Renault family | Jacques Renault | Canadian croupier, drug-runner, and bartender at the Roadhouse. Had sexual relationship with Laura Palmer | Walter Olkewicz |
| Bernard Renault | Youngest Renault brother, mules drugs over the border | Clay Wilcox | |
| Jean Renault | Oldest and most dangerous Renault brother, veteran criminal, insurance agent. | Michael Parks | |
| Blacky O'Reilly | The madame of One Eyed Jacks | Victoria Catlin | |
| Nancy O'Reilly | Blackie's sister, lover of Jean Renault | Galyn Görg | |
| Other locals | Ronette Pulaski | former employee of Horne's Department Store and One Eyed Jacks, was with Laura the night she died | Phoebe Augustine |
| Dr. Lawrence Jacoby | Eccentric former psychiatrist of Laura with an obsession for Hawaii | Russ Tamblyn | |
| Mike Nelson | Bobby's best friend, high school wrestling champ, ex-boyfriend of Donna. | Gary Hershberger | |
| Margaret Lanterman a.k.a. "The Log Lady") |
Mystic, widow who divines through a log she carries with her everywhere | Catherine E. Coulson | |
| Harold Smith | Agoraphobic horticulturist, Laura's Meals on Wheels friend | Lenny Von Dohlen | |
| Emory Battis | Store manager of Horne's Department Store, recruits girls from the perfume counter to work at One Eyed Jack's | Don Amendolia | |
| Dick Tremayne | Pretentious employee of Men's Department at Horne's, ex-lover of Lucy | Ian Buchanan | |
| The Elderly Room Service Waiter | Elderly bellhop at the Great Northern with a link to the Lodges and the Giant in particular | Hank Worden | |
| Roadhouse Singer | Angelic singer at the Roadhouse, known for her dream-like voice | Julee Cruise | |
| Mayor Dwayne Milford | Long-time mayor of Twin Peaks, has an ongoing feud with his brother Dougie | John Boylan | |
| Dougie Milford | Publisher of the Twin Peaks Gazette local newspaper, known to marry often | Tony Jay | |
| Lana Budding Milford | Seductive fiancée of Dwayne, and widow of Dougie, known to charm almost any man that's around her | Robyn Lively | |
| Outsiders | Evelyn Marsh | Rich woman who James Hurley runs into, and who is beaten by her husband | Annette McCarthy |
| Thomas Eckhardt | Former business partner of Andrew Packard, obsessively in love with Josie Packard | David Warner | |
| Jones | Thomas Eckhardt's assistant | Brenda Strong | |
| Teresa Banks | First victim, found almost exactly one year before the murder of Laura Palmer | Pamela Gidley | |
| Windom Earle | Psychotic but brilliant ex-partner of Cooper, desires the powers of the Lodges | Kenneth Welsh | |
| John Justice Wheeler | Friend of Ben Horne and later falls in love with Audrey Horne | Billy Zane | |
| Judy Swain | Foster care aid for orphan child | Molly Shannon | |
| Lodge Inhabitants | The Man from Another Place | Enigmatic dwarf, inhabitant of the Red Room. | Michael J. Anderson |
| MIKE | Spirit, former associate and now opponent of BOB, inhabits shoe salesman Phillip Gerard, "The One-Armed Man" | Al Strobel | |
| BOB | Malevolent spirit, haunts the woods and inhabits humans, killer of Laura. | Frank Silva | |
| The Giant | A supernatural giant who appears at key moments and provides Cooper with cryptic clues. | Carel Struycken | |
| Mrs Tremond/Chalfont | Link to the Lodges and intentions unclear | Frances Bay | |
| Pierre Tremond/Chalfont | Mrs Tremond/Chalfont's grandson, link to the Lodges and intentions unclear | Austin Jack Lynch/Jonathan J. Leppell |
Read more about this topic: Leo Johnson (Twin Peaks)