In Popular Culture
- A Philadelphia-based punk rock band, Sons Of Liberty, used the picture for their 2007 CD / LP "Join Or Die" (Dim Records)
- The Latin translation of Join, or Die (Jungite aut Perite) is used as the official motto of the Philadelphia Union soccer team. A snake is also featured in their logo as an allusion to this cartoon.
- A flag featuring this cartoon was prominently displayed in the opening credits of the TV miniseries John Adams and was the title for the first part of the miniseries.
- The Late Late Show host Craig Ferguson has this cartoon tattooed on the inside of his right forearm, reaching his wrist.
- An image of the Join, Or Die snake was delivered as a threat to a victim in the NCIS episode "Dead Air". It was used as a symbol for a Military At Home terrorist group.
- The cartoon is used on the cover of the novel Crooked Little Vein by comic book author Warren Ellis.
- 'Join Or Die' is the name given to the special edition of the video game Assassin's Creed 3, it being set during the American Revolutionary War.
- The cartoon is on a t-shirt worn by Justin Walker (Dave Annable) in episode 4 of season 4 ("From France with Love," 2009) of Brothers & Sisters.
- In the TNT show Falling Skies, when Pope and his gang of outlaws are corned at gunpoint by Tom Mason, Pope asks Mason for options, to which Mason replies "Join, or Die!". Part of Mason's backstory included him being a Boston University history professor who taught the American Revolution as part of his curriculum.
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