John Hartley may refer to:
- John Hartley (poet) (1839–1915), Yorkshire
- John Anderson Hartley (1844–1896), Australian educationist
- John Hartley (tennis) (1849–1935), English clergyman who won Wimbledon
- John Hartley (cricketer) (1874–1963), played for Oxford University, Sussex CCC and England
- John Hartley (academic), Professor of Cultural Studies and ARC Federation Fellow
- John Hartley, one half of duo Phil and John
- John Hartley (Published author) 6th Battalion, the Manchester Regiment in the Great War