Applications of Spontaneous Isopeptide Bond Formation
Recently, researchers have exploited spontaneous isopeptide bond formation to develop a peptide tag called SpyTag. SpyTag can spontaneously and irreversibly react with its binding partner (a protein termed SpyCatcher) through a covalent isopeptide bond. This molecular tool may have applications for in vivo protein targeting, fluorescent microscopy, and irreversible attachment for a protein microarray.
Read more about this topic: Isopeptide Bond
Famous quotes containing the words spontaneous, bond and/or formation:
“Our spontaneous action is always the best. You cannot, with your best deliberation and heed, come so close to any question as your spontaneous glance shall bring you.”
—Ralph Waldo Emerson (1803–1882)
“Man’s characteristic privilege is that the bond he accepts is not physical but moral; that is, social. He is governed not by a material environment brutally imposed on him, but by a conscience superior to his own, the superiority of which he feels. Because the greater, better part of his existence transcends the body, he escapes the body’s yoke, but is subject to that of society.”
—Emile Durkheim (1858–1917)
“That for which Paul lived and died so gloriously; that for which Jesus gave himself to be crucified; the end that animated the thousand martyrs and heroes who have followed his steps, was to redeem us from a formal religion, and teach us to seek our well-being in the formation of the soul.”
—Ralph Waldo Emerson (1803–1882)