Indole Test
In this test, the organism under consideration is grown in peptone water broth. It contains tryptophan, which under the action of enzyme tryptophanase is converted to an Indole molecule, pyruvate and carbon dioxide. The indole is then extracted from the broth by means of xylene. To test the broth for indole production, Kovac's reagent is added. A positive result is indicated by a pink/red layer forming on top of the liquid.
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