Hyphenation may refer to:
- Hyphen, a punctuation mark
- Syllabification, the splitting of words in smaller units (usually to achieve good line breaks in printed texts)
- Hyphenation algorithm, an algorithm doing automatic syllabification
- Hyphenation (analytical chemistry), the (online) coupling of analytical separation and detection technologies, e.g. Liquid chromatography–mass spectrometry (LC-MS).