Screening For Activity Against H. Nana
H. nana in mice Used because
- Human infection—easily maintained in mice
- Armed scolex similar to other pathogenic tapeworms
- Corresponds to other tapeworms in its sensitivity to standard anthelmintics
Methods
- Mature worms collected from infected mice
- Terminal gravid proglottids removed, crushed under coverslip—eggs removed
- Eggs containing hooklets (mature) counted
- 0.2 ml stock soln. containing 1000 eggs/ml given to each mouse.
- Adult worm develops — 15–17 days.
- Test drug given orally – autopsied on 3rd day
- Std. drug given
- Intestine examined under dissecting microscope for worms/ scolex
- Response – no. of mice cleared.
Read more about this topic: Hymenolepiasis
Famous quotes containing the word activity:
“Who shall set a limit to the influence of a human being? There are men, who, by their sympathetic attractions, carry nations with them, and lead the activity of the human race. And if there be such a tie, that, wherever the mind of man goes, nature will accompany him, perhaps there are men whose magnetisms are of that force to draw material and elemental powers, and, where they appear, immense instrumentalities organize around them.”
—Ralph Waldo Emerson (1803–1882)