Classification
Hydrolases are classified as EC 3 in the EC number classification of enzymes. Hydrolases can be further classified into several subclasses, based upon the bonds they act upon:
- EC 3.1: ester bonds (esterases: nucleases, phosphodiesterases, lipase, phosphatase)
- EC 3.2: sugars (DNA glycosylases, glycoside hydrolase)
- EC 3.3: ether bonds
- EC 3.4: peptide bonds (Proteases/peptidases)
- EC 3.5: carbon-nitrogen bonds, other than peptide bonds
- EC 3.6 acid anhydrides (acid anhydride hydrolases, including helicases and GTPase)
- EC 3.7 carbon-carbon bonds
- EC 3.8 halide bonds
- EC 3.9: phosphorus-nitrogen bonds
- EC 3.10: sulphur-nitrogen bonds
- EC 3.11: carbon-phosphorus bonds
- EC 3.12: sulfur-sulfur bonds
- EC 3.13: carbon-sulfur bonds
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