GUS Reporter System - Purpose

Purpose

The purpose of this technique is to analyze the activity of a promoter (in terms of expression of a gene under that promoter) either in a quantitative way or through visualization of its activity in different tissues. The technique is based on beta-glucuronidase, an enzyme from the bacterium Escherichia coli; this enzyme, when incubated with some specific colorless or non-fluorescent substrates, can transform them into coloured or fluorescent products.

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