Glutamate Dehydrogenase - Cofactors

Cofactors

NAD+(or NADP+) is a cofactor for the glutamate dehydrogenase reaction, producing α-ketoglutarate and ammonium as a byproduct.

Based on which cofactor is used, glutamate dehydrogenase enzymes are divided into the following three classes:

• EC 1.4.1.2: L-glutamate + H₂O + NAD+ 2-oxoglutarate + NH₃ + NADH + H+
• EC 1.4.1.3: L-glutamate + H₂O + NAD(P)+ 2-oxoglutarate + NH₃ + NAD(P)H + H+
• EC 1.4.1.4: L-glutamate + H₂O + NADP+ 2-oxoglutarate + NH₃ + NADPH + H+