GIT2 - Model Organisms

Model Organisms

Git2 knockout mouse phenotype
Characteristic Phenotype
Homozygote viability Normal
Fertility Normal
Body weight Normal
Anxiety Normal
Neurological assessment Normal
Grip strength Normal
Hot plate Abnormal
Dysmorphology Normal
Indirect calorimetry Normal
Glucose tolerance test Normal
Auditory brainstem response Normal
DEXA Normal
Radiography Normal
Body temperature Normal
Eye morphology Normal
Clinical chemistry Abnormal
Haematology Abnormal
Peripheral blood lymphocytes Normal
Micronucleus test Normal
Heart weight Normal
Tail epidermis wholemount Normal
Skin Histopathology Normal
Brain histopathology Abnormal
All tests and analysis from

Model organisms have been used in the study of GIT2 function. A conditional knockout mouse line, called Git2Gt(XG510)Byg was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Mice lacking Git2 had no significant defects in viability or fertility, so further tests were carried out and four significant phenotypes were reported:

  • Mutant mice had differences in their clinical blood chemistry compared to wildtype control mice.
  • Mutant male mice had a decrease in white blood cell count.
  • An increased thickness in hippocampus was observed.
  • Mutant female mice were slower to respond to heat when placed on a hotplate.

Read more about this topic:  GIT2

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