Generation
Giemsa's solution is a mixture of methylene blue, eosin, and azure B. The stain is usually prepared from commercially available Giemsa powder.
A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide. The slide is immersed in a freshly prepared 5% Giemsa stain solution for 20–30 minutes (in emergencies 5–10 minutes in 10% solution can be used), then flushed with tap water and left to dry.
Read more about this topic: Giemsa Stain
Famous quotes containing the word generation:
“You are truly the generation in the middle! You have at once aging parents as well as maturing children to cope with, and you are not granted the deference accorded age, or the indulgence given the young.”
—Helene S. Arnstein (20th century)
“One generation passeth away, and another generation cometh: but the earth abideth for ever. The sun also ariseth, and the sun goeth down, and hasteth to the place where he arose.”
—Bible: Hebrew Ecclesiastes, 1:4-5.
Ernest Hemingway took the title The Sun Also Rises (1926)
“The world is never the same as it was.... And that’s as it should be. Every generation has the obligation to make the preceding generation irrelevant. It happens in little ways: no longer knowing the names of bands or even recognizing their sounds of music; no longer implicitly understanding life’s rules: wearing plaid Bermuda shorts to the grocery and not giving it another thought.”
—Jim Shahin (20th century)