Gertrudis may refer to:
- Gertrudis Bocanegra (1765–1817), woman who fought in the Mexican War of Independence
- Gertrudis Echenique (1849–1928), First Lady of Chile between 1896 and 1901
- Gertrudis Gómez de Avellaneda (1814–1873), Cuban writer of the 19th century
- Gertrudis Gomez de Avellanda (1814–1873), Cuban born writer who lived in Spain
- Maria Gertrudis "Tules" Barceló (1800–1852), saloon owner and gambler in New Mexico
- Santa Gertrudis (disambiguation)
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