Spin Column Extraction
Gel extraction kits are available from several major biotech manufacturers for a final cost of approximately 1–2 US$ per sample. Protocols included in these kits generally call for the dissolution of the gel-slice in 3 volumes of chaotropic agent at 50 °C, followed by application of the solution to a spin-column (the DNA remains in the column), a 70% ethanol wash (the DNA remains in the column, salt and impurities are washed out), and elution of the DNA in a small volume (30 µL) of water or buffer.
Read more about this topic: Gel Extraction
Famous quotes containing the words spin, column and/or extraction:
“Words can have no single fixed meaning. Like wayward electrons, they can spin away from their initial orbit and enter a wider magnetic field. No one owns them or has a proprietary right to dictate how they will be used.”
—David Lehman (b. 1948)
“I got it: Man Without Head Kills Rich Jeweler. What an eight- column spread that’d be on the front page. Why that’s the greatest story since Lindbergh flew to Paris. Oh boy, if only it was true.”
—P. J. Wolfson, John L. Balderston (1899–1954)
“Logic is the last scientific ingredient of Philosophy; its extraction leaves behind only a confusion of non-scientific, pseudo problems.”
—Rudolf Carnap (1891–1970)