Discovery
GAB proteins were one of the first docking proteins identified in the mammalian signal transduction pathway. GAB2 along with many other adaptor, scaffold, and docking proteins, was discovered in the mid 1990s during the isolation and cloning of protein tyrosine kinase substrates and association partners. During these experiments, Nishida et al. identified a 100 kD adapter molecule with striking homology to GAB1 which they originally termed pp100. They also discovered a sequence of human cDNA that showed strong homology to GAB1 cDNA, which they termed GAB2. The group raised a polyclonal antibody against a carboxyl-terminal region of GAB2 and used it in immunoprecipitation and immunoblotting. The data gathered from these experiments indicated that pp100 is in fact GAB2.
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