Model Preparation
A comprehensive guide to creating, preparing and analysing a metabolic model using FBA, in addition to other techniques, was published by Thiele and Palsson in 2010. The key parts of model preparation are: creating a metabolic network without gaps, adding constraints to the model, and finally adding an objective function (often called the Biomass function), usually to simulate the growth of the organism being modelled.
Read more about this topic: Flux Balance Analysis
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