Measurable Parameters
This list is very long and constantly expanding.
- used for confirming diagnosis of chronic lymphocytic leukemia
- volume and morphological complexity of cells
- cell pigments such as chlorophyll or phycoerythrin
- total DNA content (cell cycle analysis, cell kinetics, proliferation, ploidy, aneuploidy, endoreduplication, etc.)
- total RNA content
- DNA copy number variation (by Flow-FISH or BACs-on-Beads technology)
- chromosome analysis and sorting (library construction, chromosome paint)
- protein expression and localization
- Protein modifications, phospho-proteins
- transgenic products in vivo, particularly the Green fluorescent protein or related Fluorescent Proteins
- cell surface antigens (Cluster of differentiation (CD) markers)
- intracellular antigens (various cytokines, secondary mediators, etc.)
- nuclear antigens
- enzymatic activity
- pH, intracellular ionized calcium, magnesium, membrane potential
- membrane fluidity
- apoptosis (quantification, measurement of DNA degradation, mitochondrial membrane potential, permeability changes, caspase activity)
- cell viability
- monitoring electropermeabilization of cells
- oxidative burst
- characterising multidrug resistance (MDR) in cancer cells
- glutathione
- various combinations (DNA/surface antigens, etc.)
- cell adherence (for instance pathogen-host cell adherence)
Read more about this topic: Flow Cytometry
Famous quotes containing the word parameters:
“What our children have to fear is not the cars on the highways of tomorrow but our own pleasure in calculating the most elegant parameters of their deaths.”
—J.G. (James Graham)