Major Intersections
| County | Location | Mile | Destinations | Notes | |
|---|---|---|---|---|---|
| Levy | Cedar Key | 0.0 | First Street | Western terminus | |
| CR 347 | |||||
| Rosewood | CR 345 | County extension of SR 345 | |||
| Otter Creek | CR 336 | ||||
| US 19 / US 98 / SR 55 | |||||
| CR 24B | |||||
| Bronson | US 27 Alt. / SR 500 |
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| CR 337 | |||||
| CR 32B | |||||
| CR 32 | |||||
| Alachua | Archer | US 27 / US 41 / SR 45 | |||
| Gainesville | I-75 / SR 93 – Tampa, Lake City | Exit 384 | |||
| SR 121 | |||||
| SR 24A / SR 226 east | |||||
| US 441 / SR 25 south | Begin US 441 concurrency | ||||
| SR 26 west / US 441 / SR 25 north | End US 441 concurrency Begin SR 26 concurrency |
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| SR 329 south / SR 20 west (Main Street) | Begin SR 20 concurrency | ||||
| SR 20 / SR 26 east / SR 24A west / SR 331 south | End SR 20/SR 26 concurrency | ||||
| SR 120 | Eastern terminus of SR 120 | ||||
| Gainesville Airport | SR 222 | ||||
| Waldo | 71.9 | US 301 / SR 200 | Eastern Terminus at interchange | ||
1.000 mi = 1.609 km; 1.000 km = 0.621 mi
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