Ent-Copalyl Diphosphate Synthase - Localization and Function

Localization and Function

ent-Copalyl diphosphate synthase has been isolated from a number of tissues in higher plants: cotyledon, hypocotyl and roots of sunflowers (Helianthus annuus) and Cucamonga manroot (Marah macrocarpus); endosperm of squash (Cucurbita maxima) and manroot (M. macrocarpus); and leaves of rice (Oryza sativa). It has been localized to the chloroplast stroma in peas (Pisum sativum) and wheat (Triticum aestivum).

The reaction catalyzed by ent-copalyl diphosphate synthase can be seen as the first committed step in gibberellin biosynthesis. Gibberellins form an important group of plant hormones, with various functions in different species and at different stages of the plant's lifetime. Disorders in gibberellin biosynthesis commonly show themselves as growth disorders, particularly as dwarfism, and some of those can be traced to reduced ent-copalyl diphosphate synthase activity. The importance of ent-copalyl diphosphate synthase in plant hormone production – primary metabolism – explains its widespread distribution both among species and among different plant tissues.

However, gibberellins are not the only phytochemicals produced from ent-copalyl pyrophosphate. A wide range of secondary metabolites, both terpenes and alkaloids, are also derived either from ent-copalyl pyrophosphate itself or from ent-kaurene or ent-kaurenoic acid, the next two intermediates on the metabolic pathway to gibberellins. Knowledge of these secondary metabolic pathways is much less extensive that that of gibberellin biosynthesis, and is often little more than conjecture.

It is known that ent-copalyl diphosphate synthase is produced by maize plants (Zea mays) in response to attack by Fusarium fungi, which suggests that it might play a role in plant defences as a precursor to phytoalexins (defensive compounds produced by the plant). Rice plants produce (at least) two different types of ent-copalyl diphosphate synthase, and only one of those participates in the production of gibberellins, suggesting again that the other is involved in the production of phytoalexins.