Enzyme Reaction
The overall reaction catalyzed by ETF-QO is as follows:
ETF-QO(red) + ubiquinone ↔ ETF-QO(ox) + ubiquinol
Enzymatic activity is usually assayed spectrophotometrically by reaction with octanoyl-CoA as the electron donor and ubiquinone-1 as the electron acceptor. The enzyme can also be assayed via disproportionation of ETF semiquinone. Both reactions are below:
Octanoyl-CoA + Q1 ↔ Q1H2 + Oct-2-enoyl-CoA
2 ETF1- ↔ ETFox + ETF2-
Read more about this topic: Electron-transferring-flavoprotein Dehydrogenase
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