DFE is a three-letter abbreviation with multiple meanings, as described below:
- Decision-feedback equalizer, a routine for reducing errors in transmitting and storing computer data
- Department for Education and Skills (2001–2007) and its successor the Department for Education, a British government department that was previously named Department for Education and Employment
- DePatie-Freleng Enterprises (1963–1981), a Hollywood-based film animation production company
- Design Focused Evaluation, an approach to the evaluation of constructive alignment in educational designs.
- Design for Environment, the concept that product design must address the product's impact upon the environment
- DFE Ascender (1979–1984), an ultralight aircraft, later known as Pterodactyl Ascender
- Dilated fundus examination, an examination of the eye using dilation fluids to open the pupil
- Distribution of Fitness Effects
- Dietary Folate Equivalent, a measure for the bioavailability of Folate
- Doctrine of foreign equivalents, a principle of US trade laws
- Double Faced Eels (formed 1999), a rock band from Latvia
- Canadian hip hop group Dragon Fli Empire
- Dunfermline Town railway station, Scotland; National Rail station code DFE
- Draft Follow and Evaluate a practice of Major League Baseball organizations