Dermatophyte Test Medium

Dermatophyte Test Medium (DTM) is a specialized agar used in medical mycology. It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator. The pH indicator is useful in distinguishing a dermatophyte fungus, which utilizes nitrogenous material for preferred metabolism, producing alkaline by-products, imparting a red color change to the medium. Typical saprotrophic fungi utilized carbohydrates in the medium producing acidic by-products and no red color change.

Growth media / agar plates
Selective media
Gram positive
Actinobacteria Mycobacterium tuberculosis (Lowenstein-Jensen medium, Middlebrook 7H9 Broth, Middlebrook 7H10 Agar, Middlebrook 7H11 Agar) · Mycoplasma pneumoniae (Eaton's agar)
Firmicutes Corynebacterium diphtheriae (Hoyle's agar) · Enterococcus (Bile esculin agar) · Lactobacillus (MRS agar) · Staphylococcus (Mannitol salt agar)
Gram negative
Alphaproteobacteria Brucella abortus (Brucella agar)
Betaproteobacteria Neisseria (Thayer-Martin agar)
Gammaproteobacteria Bordetella (Bordet-Gengou agar) · Enterobacteriaceae (VRBD agar) · Haemophilus influenzae/Legionella pneumophila (Buffered charcoal yeast extract agar) · Pseudomonas aeruginosa (Cetrimide agar) · Salmonella (XLT agar) · DCA agar · Salmonella/Shigella (XLD agar)
Differential media Lactose fermenting gram negative (MacConkey agar/Sorbitol-MacConkey agar, Eosin methylene blue) · Hektoen enteric agar · sulfur (Bismuth sulfite agar)
Fungal media Dermatophyte test medium · Potato dextrose agar · Sabouraud agar
Nonselective media Chocolate agar · Nutrient agar · Plate count agar
Other/ungrouped media Cysteine lactose electrolyte deficient agar · Cystine tryptic agar · Endo agar · LIA slant · Müller-Hinton agar/PNP agar · R2a agar · Simmons' citrate agar · Trypticase soy agar · TSI agar

M: BAC

bact (clas)

gr+f/gr+a (t)/gr-p (c)/gr-o

drug (J1p, w, n, m, vacc)


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