Background Mutation Rates
It is reported that when no double stranded breaks are generated, the number of cells that lose the CORE cassette in the absence of a targeting oligonucleotide is less than one transformant per 107 viable cells. In contrast, this number increases up to 100 transformants per 107 viable cells when a double stranded break is generated. In a diploid, the increased background mutation rates occur due to homologous recombination with the homologous chromosome decreasing targeted transformation events to only 4% of the total transformants.
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