DBT may refer to:
- Danish Board of Technology, a technology assessment institution in Denmark
- DBT (gene)
- DBT Online Inc. a US data mining company
- Department of Biotechnology, India
- .dbt, the extension of a DBase file format
- Design-basis tornado within a Design basis accident of a nuclear facility
- Deutscher Bundestag, the lower house of the bicameral parliament of Germany
- Dialectical behavior therapy, a psychotherapy for psychiatric illnesses such as borderline personality disorder
- Dibenzothiophene, a chemical compound
- Dibutyltryptamine, a psychedelic drug
- Drive-By Truckers, an alt-country/rock band
- Dynamic Binary Translation, a technique often used by Emulators