In enzymology, a crotonoyl- hydratase (EC 4.2.1.58) is an enzyme that catalyzes the chemical reaction
- (3R)-3-hydroxybutanoyl- but-2-enoyl- + H2O
Hence, this enzyme has one substrate, ]], and two products, ]] and H2O.
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (3R)-3-hydroxybutanoyl- hydro-lyase (but-2-enoyl--forming). Other names in common use include (3R)-3-hydroxybutanoyl- hydro-lyase, beta-hydroxybutyryl acyl carrier protein dehydrase, beta-hydroxybutyryl acyl carrier protein (ACP) dehydrase, beta-hydroxybutyryl acyl carrier protein dehydrase, enoyl acyl carrier protein hydrase, crotonyl acyl carrier protein hydratase, 3-hydroxybutyryl acyl carrier protein dehydratase, beta-hydroxybutyryl acyl carrier, and protein dehydrase. This enzyme participates in fatty acid biosynthesis.