Cootie can refer to:
- Head lice (slang)
- Cooties, a fictional disease which affects children in their early years
- Cootie (game), a game in which each player assembles a bug from plastic parts upon the roll of a die
- Beetle (game), the same game as above, played with pencil and paper
- Telegraph key, alternate name for a sideswiper manual telegraph key
- Cootie Williams, American jazz trumpeter