Advantages
The primary advantage of the Columnmaster 4000 is its ability to deliver liquid to the column without disturbing the column bed. Such disturbances could lead to unacceptable mixing of two column washes, resulting in decreased purity of the sample on the column. Because the Columnmaster delivers water only as fast as the solute level is depleted, excess liquid is not delivered before it in needed, minimizing backflow and dilution. Another key advantage to using the Columnmaster 4000 is the ability of the researcher to leave the column for extended periods of time without the concern of overflow. The user can control the water level based on the positioning of the tubing. Multiple column volumes can be added based on the amount of liquid the user puts into the source vessel, which they can do all at once (as opposed to in small aliquots, as was previously necessary to ensure proper switching between washes.)
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