Columbiana may refer to:
In U.S. places:
- Columbiana, Alabama
- Columbiana, Ohio
- Columbiana High School
- Columbiana Exempted Village School District, a school district in Ohio
- Columbiana County, Ohio
- Columbiana County Airport, an airport in Ohio
In botany:
- Arctostaphylos columbiana or hairy manzanita, a plant species native to North America
- Calatola columbiana, a species of plant in the Icacinaceae family endemic to Colombia
- Clematis columbiana, a species of flowering plant in the buttercup genus Clematis
- Crataegus columbiana, several species of Hawthorn plant native to North America
- Lewisia columbiana or Columbian lewisia, a species of flowering plant in the purslane family
- Wolffia columbiana or Columbian watermeal, a perennial aquatic plant in the Duckweed family
In zoology:
- Dictyna columbiana, a Dictynidae species of spider
- Nucifraga columbiana or Clark's nutcracker, a species of passerine bird in the family Corvidae
- Sicalis columbiana or Orange-fronted yellow-finch, a species of bird in the Emberizidae family
- Xylophanes columbiana, a species of moth in the family Sphingidae