Steps in Molecular Cloning
In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) Selection of organisms containing recombinant DNA, (7) Screening for clones with desired DNA inserts and biological properties.
Read more about this topic: Clone (genetics)
Famous quotes containing the words steps in, steps and/or cloning:
“Now Morn her rosy steps in th’ eastern clime
Advancing, sowed the earth with orient pearl.”
—John Milton (1608–1674)
“He who, from zone to zone,
Guides through the boundless sky thy certain flight,
In the long way that I must tread alone,
Will lead my steps aright.”
—William Cullen Bryant (1794–1878)
“Language is as real, as tangible, in our lives as streets, pipelines, telephone switchboards, microwaves, radioactivity, cloning laboratories, nuclear power stations.”
—Adrienne Rich (b. 1929)