Chuquisaca may refer to:
- Spanish colonial city, and its surrounding province, founded with the name of Ciudad de la Plata de la Nueva Toledo in 1538 (later variously called La Plata, Chuquisaca, Charcas and Sucre) in Upper Peru; seat of the Real Audiencia of Charcas and later of an intendancy established in 1782 within the Viceroyalty of the Río de la Plata
- Bolivia's (smaller) republican Chuquisaca Department