History
The 3C methodology was originally developed by Dekker in the Kleckner lab in 2002 at Harvard University and it aimed at identifying, locating and mapping physical interactions between genetic elements located throughout the human genome. This technology would give beneficial insights into the complex interplay of genetic factors that contribute to such debilitating disorders such as cancer, Duchenne muscular dystrophy (DMD), Rett syndrome and Alzheimer's disease.
3C is based on proximity ligation, which had been used previously to determine circularization frequencies of DNA in solution, and the effect of protein-mediated DNA bending on circularization. Seyfred and colleagues developed proximity ligation in nuclei: restriction enzyme digestion of unfixed nuclei and ligation in situ without diluting the chromatin, which they termed the "Nuclear Ligation Assay" (Cullen et al. Science. 1993 Jul 9;261(5118):203-6; Gothard LQ et al. Mol Endocrinol. 1996 Feb;10(2):185-95).
Read more about this topic: Chromosome Conformation Capture
Famous quotes containing the word history:
“History is not what you thought. It is what you can remember. All other history defeats itself.
In Beverly Hills ... they don’t throw their garbage away. They make it into television shows.
Idealism is the despot of thought, just as politics is the despot of will.”
—Mikhail Bakunin (1814–1876)
“When the coherence of the parts of a stone, or even that composition of parts which renders it extended; when these familiar objects, I say, are so inexplicable, and contain circumstances so repugnant and contradictory; with what assurance can we decide concerning the origin of worlds, or trace their history from eternity to eternity?”
—David Hume (1711–1776)
“We know only a single science, the science of history. One can look at history from two sides and divide it into the history of nature and the history of men. However, the two sides are not to be divided off; as long as men exist the history of nature and the history of men are mutually conditioned.”
—Karl Marx (1818–1883)