A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which together constitute some portion of the transcriptome of the organism. cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism. Similarly, tissue specific cDNA libraries can be produced. In eukaryotic cells the mature mRNA is already spliced, hence the cDNA produced lacks introns and can be readily expressed in a bacterial cell. While information in cDNA libraries is a powerful and useful tool since gene products are easily identified, the libraries lack information about enhancers, introns, and other regulatory elements found in a genomic DNA library.
Read more about CDNA Library: CDNA Library Construction, CDNA Library Uses, How Do We Clone CDNA Molecules?
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“The fear of failure is so great, it is no wonder that the desire to do right by one’s children has led to a whole library of books offering advice on how to raise them.”
—Bruno Bettelheim (20th century)