Dual Activity
An entirely new perspective on CDK7 function was opened when CDK7 was identified as a subunit of transcription factor IIH (TFIIH) and shown to phosphorylate the carboxy-terminal domain (CTD) of RNA polymerase II (RNAPII). TFIIH is a multiprotein complex required not only for class II transcription but also for nucleotide-excision repair. Its associated CTD-kinase activity is considered important for the promoter-clearance step of transcription, but the precise structural consequences of the phosphorylation of the CTD remain the subject of debate. Cyclin H and MAT1 are also present in TFIIH, and it is not known what, if anything, distinguishes the TFIIH-associated form of CDK7 from the quantitatively predominant free form. Whether CDK7 really displays dual-substrate specificity remains to be further explored, but there is no question that the CDK7-cyclin H-MAT1 complex is able to phosphorylate both the T-loop of CDKs and the YSPTSPS (single-letter code for amino acids) repeats of the RNAPII CTD in vitro.
Read more about this topic: CDK7 Pathway
Famous quotes containing the words dual and/or activity:
“Thee for my recitative,
Thee in the driving storm even as now, the snow, the winter-day
declining,
Thee in thy panoply, thy measur’d dual throbbing and thy beat
convulsive,
Thy black cylindric body, golden brass and silvery steel,”
—Walt Whitman (1819–1892)
“The animal is one with its life activity. It does not distinguish the activity from itself. It is its activity. But man makes his life activity itself an object of his will and consciousness. He has a conscious life activity. It is not a determination with which he is completely identified.”
—Karl Marx (1818–1883)