Applications
One of CASY technology applications is electronic cell counter for determining cell number and their viability in a sample. The equipment is shown as Fig. 3 and the Fig. 4 states the result, including the total cell count, as well as the percentage of debris, living cells and dead cells, displayed on the screen of the cell counter.
Lindl et al. (2005) has compared the CASY technology to two standard methods for cell viability measurement, including the neutral red uptake and MTT assay. They found that the most sensitive IC50 values, which were the closest to those in the literature, were performed by this electronic cell counter. Some toxicants in these experiments by using chemical methods would affect the mechanisms of the assays. So, the results would become invalid. However, for the electronic cell counter, it can not only monitor all the cells changes, even the cell necrosis, by various toxicants types and concentration, but also a complex mixture of toxicants in the cell culture. It would be seen that the progress changes of dying cells can be detected as well. On the other hand, all the results from electric cell counter could be transferred to the computers with common spreadsheet programs. No other specific software would be set up to every computer to obtain the result.
Read more about this topic: CASY Cell Counting Technology