Capacitation - Capacitation

Capacitation

Capacitation involves the destabilisation of the acrosomal sperm head membrane allowing greater binding between sperm and oocyte. This change is facilitated by the removal of steroids (e.g. cholesterol) and non-covalently bound epididymal/seminal glycoproteins. The result is a more fluid membrane with an increased permeability to Ca2+.

An influx of Ca2+ produces increased intracellular cAMP levels and thus, an increase in motility. Hyperactivation coincides with the onset of capacitation and is the result of the increased Ca2+ levels. The tripeptide FPP (fertilization promoting peptide) produced by the male is essential for capacitation (high levels of FPP prevent capacitation, the proper concentration occurs after ejaculation in the female reproductive tract where the concentration drops after mixing with vaginal secretions and/or becomes less active due to the pH of the vagina). It has a synergistic stimulatory effect with adenosine that increases adenylyl cyclase activity in the sperm. FPP is found in the seminal fluid (FPP produced in prostate gland), and comes into contact with the spermatozoa upon ejaculation.