Blue White Screen
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector. The vector is then transformed into competent cell (bacteria), and the competent cells are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
Read more about Blue White Screen: Background, Molecular Mechanism, Practical Considerations
Famous quotes containing the words blue, white and/or screen:
“Two wooden tubs of blue hydrangeas stand at the foot of the stone steps.
The sky is a blue gum streaked with rose. The trees are black.
The grackles crack their throats of bone in the smooth air.
Moisture and heat have swollen the garden into a slum of bloom.
Pardie! Summer is like a fat beast, sleepy in mildew....”
—Wallace Stevens (1879–1955)
“I’ve often wondered why she laughed
On thinking why I wondered so;
It seemed such waste that long white hands
Should touch my hands and let them go.”
—Allen Tate (1899–1979)
“Between two evils, I always pick the one I never tried before.”
—Raoul Walsh, U.S. screen writer. Frisco Doll (Mae West)