Blue White Screen
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector. The vector is then transformed into competent cell (bacteria), and the competent cells are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
Read more about Blue White Screen: Background, Molecular Mechanism, Practical Considerations
Famous quotes containing the words blue, white and/or screen:
“Oh, Scott, for people like you and me the world can be a wonderful place. The sky’s as blue as it is for the giants, the friends are as warm.”
—Richard Matheson (b. 1926)
“...there was the annual Fourth of July picketing at Independence Hall in Philadelphia. ...I thought it was ridiculous to have to go there in a skirt. But I did it anyway because it was something that might possibly have an effect. I remember walking around in my little white blouse and skirt and tourists standing there eating their ice cream cones and watching us like the zoo had opened.”
—Martha Shelley, U.S. author and social activist. As quoted in Making History, part 3, by Eric Marcus (1992)
“We like the chase better than the quarry.... And those who philosophize on the matter, and who think men unreasonable for spending a whole day in chasing a hare which they would not have bought, scarce know our nature. The hare in itself would not screen us from the sight of death and calamities; but the chase, which turns away our attention from these, does screen us.”
—Blaise Pascal (1623–1662)