Blue White Screen
The blue-white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. DNA of interest is ligated into a vector. The vector is then transformed into competent cell (bacteria), and the competent cells are grown in the presence of X-gal. Cells transformed with vectors containing recombinant DNA will produce white colonies; cells transformed with non-recombinant plasmids (i.e. only the vector) grow into blue colonies.
Read more about Blue White Screen: Background, Molecular Mechanism, Practical Considerations
Famous quotes containing the words blue, white and/or screen:
“The moon is my mother. She is not sweet like Mary.
Her blue garments unloose small bats and owls.”
—Sylvia Plath (1932–1963)
“Kind Sir: Lost and of your same kind
I have turned around twice with my eyes sealed
and the wood were white and my night mind
saw such strange happenings, untold and unreal.”
—Anne Sexton (1928–1974)
“Every obstruction of the course of justice,—is a door opened to betray society, and bereave us of those blessings which it has in view.... It is a strange way of doing honour to God, to screen actions which are a disgrace to humanity.”
—Laurence Sterne (1713–1768)